Levels of MDA and SOD in acute gastric mucosal injury in rats exposed to positive acceleration

Abstract

AIM: To determine the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in acute gastric mucosal injury in rats exposed to positive acceleration (+Gz), observe the impact of +Gz exposure on gastric mucosal injury, and clarify the role of oxygen free radicals in this process.METHODS: Thirty male SD rats were randomly divided into three groups: A (ethanol), B (ethanol with +5Gz exposure), and C (ethanol with +10Gz exposure). All rats were intragastrically given ethanol (0.4 mL/100 mg) after 24 h of fasting and water deprivation for 12 h. One hour after ethanol administration, group A did not undergo +Gz, while groups B and C were continuously exposed to +5Gz and +10Gz for 3 minutes, respectively. Immediately after +Gz exposure, gastric tissue samples were taken to observe gastric mucosal injury by light microscopy. The indexes of gastric mucosal injury were calculated, and the contents of MDA and SOD in the gastric mucosa were determined by ELISA. RESULTS: Gastric mucosal injury was observed in all the groups, and gastric mucosal injury was most severe in group C, followed by groups B and A. Group A had mildest gastric mucosal injury. Group B showed visible gastric mucosal congestion, edema and scattered bleeding spots, and light microscopy revealed acute inflammatory cell infiltration. The indexes of gastric mucosal injury were significantly higher in group B than in group A (naked eye: 23.654 ± 9.678 vs 11.410 ± 3.742; light microscopy: 5.000 ± 1.054 vs 3.800 ± 1.399; both P 0.05). Group C had heaviest gastric mucosal injury. Diffuse gastric mucosal congestion, edema, erosion and many bleeding spots were visible, and light microscopy revealed disorderly arranged gland structure, interstitial congestion, erosion, and acute inflammatory cell infiltration. The indexes of gastric mucosal injury were significantly higher in group C than in groups A and B (naked eye: 49.080 ± 10.254, light microscopy: 9.400 ± 2.011; all P 0.05). Compared with group A, the content of MDA in the gastric mucosa did not rise significantly in group B (0.255 ± 0.074 vs 0.235 ± 0.044, P 0.05); however, MDA content in group C (0.376 ± 0.084) was significantly higher than those in groups A and B (both P 0.05). The content of SOD in the gastric mucosa was significantly lower in group C than in groups A and B (8.852 ± 1.001 vs 10.000 ± 1.067, 10.694 ± 0.965, P 0.05),although no significant difference was observed between the latter two groups (P 0.05). CONCLUSION: +Gz exposure aggravates acute gastric mucosal injury in rats possibly by altering the contents of MDA and SOD in gastric tissue.