Effects of the Neonicotinoid Imidacloprid on the Feeding Behavior of Melipona quadrifasciata anthidioides Lep.

Luiz Guilherme De Lima Costa,Angel Roberto Barchuk,Isabel Ribeiro Do Valle Teixeira

doi:10.18406/2316-1817v12n120201411

Abstract

Even though meliponines are among the greatest pollinating agents in Neotropical ecosystems, the populations of these bees are in decline due to anthropic actions, such as the indiscriminate use of pesticides. Neonicotinoids, such as Imidacloprid, are used in many crops and have been shown to be one of the pesticides groups with the most damaging effects on the health of bee colonies. The goals of this work were (1) to evaluate the ability of Melipona quadrifasciata, a native Brazilian stingless bee species, to detect the presence of Imidacloprid in artificial diets, and (2) to evaluate how this insecticide may affect their behavior. For this purpose, behavioral experiments were carried out in observation arenas where artificial flowers containing different concentrations of Imidacloprid (0%, 1%, 5%, 10%, and 20%) were made available to forager bees and their choice and behavior were registered. Our results showed that bee choices were not affected by the different concentrations of Imidacloprid, even at extremely high doses in the flowers (20%). It was also found that the presence of this insecticide interfered with the communication between individuals as a result of reduced antennation events. Both findings, the apparent inability of bees to detect the presence of the insecticide and the change in behavior caused by the exposition to the volatilized product, may rapidly lead to colony losses in nature.

Highlights

Seventy-five percent of all known meliponine species are found in the Neotropical region (COSTA et al, 2003)
Considering that the detection and possible rejection of Imidacloprid in plant parts collected by bees would prevent bees from getting toxins in their organism, this study aimed to evaluate the behavior of M. quadrifasciata anthidioides Lepeletier, 1836, during foraging and its ability to detect the presence of Imidacloprid in artificial diets
We found that Mandaçaia bees have the ability to distinguish diets with different compositions, the presence of Imidacloprid does not influence their food preference behavior

Summary

Introduction

Seventy-five percent of all known meliponine species are found in the Neotropical region (COSTA et al, 2003). “Mandaçaia” Melipona quadrifasciata Lepeletier (1836) are natives to the Brazilian states of Mato Grosso do Sul, Minas Gerais, Paraná, Rio Grande do Sul, Rio de Janeiro, Santa Catarina and São Paulo, but they appear in some regions of Argentina and Paraguay. This species stands out from other stingless bees due to its long daily foraging period, starting right after sunrise and stopping just before sunset (AIDAR, 2011), which allows a large number of floral visits. Scientific studies that seek to investigate environmental effects that influence the survival and reproduction of bees are increasingly necessary

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Conclusion