Abstract
Inhibitory effects of tannins on copper (II)-catalyzed autoxidation of ascorbic acid were examined. Tannic acid JP and geraniin were shown to be much more effective than the related polyphenols of smaller molecules, gallic acid and pyrogallol etc. Geraniin showed a stronger antioxidative effect than tannic acid JP. This result is attributable to the possible chelation between the potential triketone moiety in geraniin, and the metal ion. The quantitative analysis of ascorbic acid in the reaction mixture was carried out by dual-wavelength thin-layer chromatography-densitometry which gave reproducible results in the presence of polyphenols.
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