Effects of the Diaomo-Zhibeng liquid on the expression of Bcl-2/Bax mRNA and their protein in vitro human endometrial glandular epithelial cells

Abstract

Objective To observe the effects of the Diaomo-Zhibeng liquid on the expression of Bcl-2/Bax mRNA and their protein in vitro endometrial glandular epithelial cells in patients of endometrial hyperplasia Methods The endometrial tissue in patients with the pathological diagnosis of endometrial hyperplasia were collected. After isolation, culture and identification, the endometrial cells were divided into four groups: low dosage group(12.5 μg/ml), middle dosage group(25 μg/ml), high dosage group(50 μg/ml) and blank control group at randomaccording to the random number table. Three duplicate samples were set for each group. After 48h, the expression of Bcl-2 and Bax were detected by RT-PCR and Western-blot. Results Compared with the blank control group, the expression of Bcl-2 mRNA (1.51 ± 0.07, 1.09 ± 0.06, 0.93 ± 0.07 vs. 1.92 ± 0.08) and protein (0.91 ± 0.02, 0.72 ± 0.03, 0.62 ± 0.03 vs. 1.28 ± 0.02) significantly decreased, while the expression of Bax mRNA (5.73 ± 0.37, 8.00 ± 0.23, 10.72 ± 0.40 vs. 3.27 ± 0.34) and protein (0.45 ± 0.02, 0.63 ± 0.02, 0.78 ± 0.03 vs. 0.32 ± 0.02) sifnificantly promoted in low dosage group, medium dosage group and high dosage group of Diaomo-Zhibeng liquid (P<0.05). Compared with low dosage group, the expression of Bcl-2 mRNA and protein significantly decreased in middle and high dose group (P<0.05), but Bax mRNA and protein significantly increased (P<0.05). Conclusions The Diaomo-Zhibeng liquid can increase the susceptibility of epithelial cells to apoptosis, regulate the balance of cell proliferation and apoptosis tends to apoptosis, have the effect of accelerating cells apoptosis. Key words: Endometrial hyperplasia; Apoptosis; Genes, bcl-2; bcl-2-Associated X protein; Diaomo-Zhibeng Liquid