Abstract
Ten steroids have been compared for their ability to modify the rate of uptake of acridine orange by rat liver and by rat liver lysosomes in vivo . The short-term effects of the ten steroids on the specific activity of a lysosomal enzyme, β-N-acetylglucosaminidase, were also compared. Five of the ten steroids were administered as tritium-labelled compounds and the concentration of steroids or metabolites was measured in rat liver and liver lysosomes at 2.5 h and 3.75 h after administration. Cortisone acetate, etiocholanolone (5β-androstan-3α-ol-17-one) and testosterone accelerate and increase the uptake of acridine orange by rat liver lysosomes. Deoxycorticosterone, corticosterone, triamcinolone (9α -fluoro-11β, 16α, 17, 21-tetrahydroxy-pregna-1, 4-diene-3, 20-dione), estra-diol-17β and progesterone appear to inhibit the uptake of acridine orange by rat liver lysosomes at 2.5 hours. Cortisol and dexamethasone (9α-fluoro-11β, 17, 21-trihydroxy-16α-methyl-pregna-1, 4-diene-3, 20-dione) had little effect. All steroids with the exception of etiocholanolone and deoxycorticosterone increase the specific activity of β-N-acetylglu-cosaminidase in the lysosomal fraction at 2.5 h. Nope of the effects at 2.5 h are due to lowered protein levels. Lysosomal concentrations of radioactivity following the administration of tritiated steroids were greatest for the glucocorticoids, corticosterone and cortisol. Estradiol-17β progesterone and testosterone showed much lower concentrations of radioactivity in isolated lysosomes. Most of the lysosomal radioactivity (73–96%) was associated with the soluble fraction of the disrupted lysosomes.
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