Abstract
Disks cut from dead strawberry leaves (autoclaved or air-dried) were inoculated with a conidial suspension of Botrytis cinerea (10(6) conidia per ml) and incubated at various temperatures (T) ranging from 5 to 30 degrees C. Sporulation (conidia per cm2) on leaf disks was determined after exposure to wetness durations (W) of 3 to 11 days. Optimum temperature for sporulation was between 17 and 18 degrees C at all wetness durations. Sporulation levels of 10(5) to 10(7) conidia per cm2 were observed between 15 and 22 degrees C, after 7 days of continuous wetness. As temperature increased or decreased from the optimum, sporulation decreased for the same wetness durations. Very little sporulation was observed at 25 degrees C and no sporulation was observed at 30 degrees C. Logarithmic polynomial models best described the effect of T and W on sporulation of B. cinerea on dead strawberry leaf tissue. Coefficients of determination for data from all repetitions of the experiments were at least 0.81. The latent period of B. cinerea on dead leaf tissue was longest at the lowest temperature (6 to 7 days at 5 degrees C) and decreased to <3 days as temperature increased to the optimum (15 to 22 degrees C). Interrupted wet and dry periods of 5. 12, and 24 h directly affected sporulation. Total hours of wetness and the duration of individual wet periods had the greatest effect on the amount of inoculum produced at 20 degrees C.
Published Version
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