Effects of technical circumstances of the experiment on some electrophysiological parameters in rats

Abstract

Event Abstract Back to Event Effects of technical circumstances of the experiment on some electrophysiological parameters in rats András Papp1*, Marietta Morvai1, Viktória Nagy1 and Anikó Takács1 1 University of Szeged, Department of Public Health, Hungary Electrophysiological experiments are often performed in anesthetized animals, with parts of the nervous system prepared for access and recording. In such state, mechanisms upkeeping normal physiological state cannot be fully relied upon. Anesthesia, if deep enough for surgical preparation and undisturbed recording, largely reduces the animal’s ability to maintain normal body temperature. This may result in hypothermia, causing alteration of electrophysiological parameters (spectrum of electrocorticogram, nerve conduction velocity etc.). Another problem is represented by the effects of the anesthetic itself. In this work, cortical evoked potentials, electrocorticogram, and tail nerve action potential were recorded from rats using under various anesthetics and varied body temperature. By sudden cooling the support pad of the rat fixing apparatus to ca. 15 °C and gradual rewarming to 37°C, the rectal temperature was lowered to ca. 30°C then restored. The relative power of beta band within the spontaneous cortical activity increased, and that of delta and theta band decreased, on cooling. Of the cortical response evoked by whisker stimulation, both latency and amplitude increased. All cortical parameters showed good linear correlation with rectal temperature whereby the fitted straight line was mostly steeper in anesthesia with ketamine-xylazine than with urethane, indicating more disturbed thermoregulation. Conduction velocity of the tail nerve correlated better with the temperature of the support pad than with rectal temperature. The somatosensory cortical response evoked by whisker stimulation in rats anesthetized by chloral hydrate had a profoundly different shape compared to responses under urethane or ketamine-xylazine. The spread of excitation from the sensory to the motor area, evaluated by comparing the response from the two foci, was also different with different anesthetics. Stability and repeatebility of technical circumstances is crucial indeed for achieving reliable results. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Abstracts Citation: Papp A, Morvai M, Nagy V and Takács A (2010). Effects of technical circumstances of the experiment on some electrophysiological parameters in rats. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00255 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 May 2010; Published Online: 05 May 2010. * Correspondence: András Papp, University of Szeged, Department of Public Health, Szeged, Hungary, ppp@puhe.szote.u-szeged.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers András Papp Marietta Morvai Viktória Nagy Anikó Takács Google András Papp Marietta Morvai Viktória Nagy Anikó Takács Google Scholar András Papp Marietta Morvai Viktória Nagy Anikó Takács PubMed András Papp Marietta Morvai Viktória Nagy Anikó Takács Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.