Effects of Tanshinone on Hemodynamics and Expression of NF- B and Myeloperoxidase in Rats with Spinal Cord Ischemia-Reperfusion Injury

Abstract

Spinal cord ischemia-reperfusion injury (SCII) can cause spinal nerve injury and loss of function. Tanshinone IIA has antioxidant activity. However, the effect of tanshinone on SCII rats remains unclear. SD rats were randomly divided sham operation group (sham group), SCII group and tanshinone group. Heart rhythm (HR), mean blood pressure (MAP), and myocardial oxygen consumption index (RPP) hemodynamic parameters were analyzed. Rat neurological function scores were performed by the Tarlov method. The glutamate detection kit was used to detect the content of glutamate, superoxide dismutase (SOD) and myeloperoxidase (MPO). NF-κ B protein expression was assessed by Western blot. The expressions of tumor necrosis factor-α (TNF-α) and interleukin6 (IL-6) were analyzed by ELISA. In SCII group, HR, MAP, RPP, and neurological function score as well as SOD activity were significantly decreased with increased MPO, glutamate, TNF-α and IL-6 secretion as well as elevated NF-κ B expression compared with sham group (P < 0 05). After tanshinone treatment, HR, MAP, RPP, and neurological function score as well as SOD activity were all significantly increased along with decreased MPO, glutamate, TNF-α and IL-6 secretion, as well as reduced NF- κB expression compared with SCII group (P < 0 05). Tanshinone can improve the oxidative stress, reduce the secretion of inflammatory factors, and improve hemodynamics which is possibly through regulating NF-κ B expression.