Abstract
Objective To explore the role of macrophage in the development of rheumatoid arthritis(RA) by systemic depletion of macrophages with Clodronate liposomes(Clo-lip). Methods The depletion of macrophages in the spleens of mice was detected by hematoxylin and eosin(HE) staining and immunohistochemistry.30 BALB/c mice were randomly divided into three groups: the RA-model mice were established as control A group; RA modelmice were established as PBS-lip B group and Clo-lip C group, which were administered with lips.The appearance of the mouse pawswere observed and degree of arthritis were recorded by the histological features and the degree of the infiltrating inflammatory cells. Results Compared with the control group, horseshoe-shaped nuclei, multi-core large cells, CD68 macrophage were few determined by HE staining(t=15.46, P<0.05) and immunohistochemistry(t=14.70, P<0.05)in C group.The score of arthritis degree was lower in C group than that in other two groups.The difference was statistically significant(t=8.578, P<0.05). HE staining showed that there was no inflammatory cell infiltration in articular cavity in the mice of clo-lip group. Conclusions Systemic depletion of macrophage with Clo-lip affects the occurrence of RA, indicating that macrophage plays a key role in the development of RA. Key words: Rheumatoid arthritis; Macrophage; Clodronate liposomes; Mouse
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