Study on the rheumatoid arthritis mouse model induced by glucose-6-phosphate isomerase

Abstract

Objective To establish the rheumatoid arthritis (RA) mouse model induced by glucose-6-phosphate isomerase (GPI), and explore the mechanism of GPI in RA. Methods Totally 36 DBA/1 mice were randomly divided into three groups: test group (injection GPI), positive group (injection of bovine collagen Ⅱ), and negative group (saline). The rates and changes of weight were observed. The score of the arthritis, the ankle histopathological changes and serum GPI content were detected. Results Toes swollen slightly, joint swelling, deformity and accompanied by block were appeared at 35th day in the test group. Compared to the control group, the rates and changes of weight in test group showed a significant difference (P<0.05). The score of arthritis was showed by ±s. Compared to the negative group, the test group and positive group were showed significant difference (P<0.05). A lot of lower synovial lining exudate macrophages, fibroblasts, and other inflammatory cells were increased in the test group. The GPI content in the test group [(0.39±0.11)μg/ml] was significantly higher than the negative group [(0.10±0.06)μg/ml, P<0.05]. Conclusions GPI could induce rheumatoid arthritis in mice. It provides the experimental basis to diagnose RA. Key words: Glucose-6-phosphate isomerase/AE/ME; Arthritis, rheumatoid/CI/ME; Disease models, animal