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Abstract
Synthetic LH-RH (1 μg/ml) in vitro increased cyclic AMP concentration of rat anterior pituitary within 1 min and HL release into the medium after 15 min incubation. The minimum effective dose of synthetic LH-RH on cyclic AMP formation was 10 ng/ml. Data obtained using synthetic analogs of LH-RH indicated that the N-terminal amino acid and the C-terminal carboamide group of synthetic LH-RH are necessary for biologic activity.
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