Effects of synbiotic (Biomin IMBO) on haematological and immunological components of Russian sturgeon, Acipenser guldenstadti

Abstract

The present study was carried out to evaluate the effects of synbiotic (Biomin IMBO) on haematological (white blood cell (WBC) and red blood cell (RBC) counts, haemoglobin (Hb) concentration, haematocrit (Hct), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular haemoglobin concentration (MCHC)) and immunological (immunoglobulin (IgM) and lysozyme activity) components of Russian sturgeon, Acipenser guldenstadti. Five experimental treatments and one control group with three replicates were considered. In treatment groups, fish were fed with diet containing 1 (T1), 1.5 (T2), 2 (T3), 2.5 (T4) and 3 (T5) g synbiotic/kg diet. In control group, fish were fed without supplement of synbiotic. Blood sampling for haematological and immunological studies was done at days 20, 40 and 60 after the beginning of the experiment. Based on our results, synbiotic supplement had no significant effects on values Hb, Htc, MCV and MCHC compared to control group (P > 0.05). Also, in all experimental treatments, these parameters did not considerable changes during the course of the experiment (P > 0.05). The effect of synbiotic supplement on MCH was not significant (P > 0.05); however, this value was higher in synbiotic supplement treatments compared to control group. MCH had no significant changes during the period of the experiment in all experimental groups (P > 0.05). In all synbiotic treatments, the values of WBC count, neutrophils, monocytes and eosinophils were higher than control group (P 0.05). The values of RBCs in T4 and T5 showed an increasing change over the course of the experiment (P 0.05).