Effects of Surgical Antiseptics on Streptofibrinolysin.

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To determine the effects of surgical antiseptics on the fibrinolytic activity of Streptococcus liemolyticus tests were made of: (a) the effects of sub-bacteriostatic concentrations of the commoner antiseptics on the rate of fibrinolysin-production by glucose veal-infusion broth cultures of S. liemolyticus, and (b) the effects of certain selected concentrations of the same antiseptics on the coagulation and autolysis of human, fibrin and on the liquefaction of isolated human fibrin by streptococcal cultures (or filtrates). To determine the effects of antiseptics on fibrinolysin-production sub-bacteriostatic dilutions of the commoner antiseptics (Table I) were made in routine dextrose veal-infusion broth. Ten cc. of each of the resulting antiseptic-broth mixtures were inoculated with one loopful of a 24-hour broth culture of S. hemolyticus and incubated at 37°C. for 24 hours. The total bacterial population and the total number of fibrinolytic units per cc. were then determined for each subculture. (For technics see previous paper.) Typical data are recorded in Fig. 1. The table shows a partial inhibition of lysin-production in sub-bacteriostatic concentrations of zonite, the lysin-production varying proportionally with the rate of streptococcal proliferation. The same parallelism between bacterial count and lytic titer was noted with all other antiseptics (Table I) thus far tested by us. This parallelism tends to confirm our previous conclusion that there is a quantitative linkage between the rate of cell-division and the rate of fibrinolysin-production with S. liemolyticus. To study the effects of the same antiseptics on coagulation and autolysis of human fibrin, solutions of fibrinogen and thrombin were prepared by the technic of Tillett and Garner. Serial dilutions of the stock antiseptic solutions were made in the standard fibrinogen-solution and coagulation attempted by the addition of a routine dose of thrombin.