Abstract
The effects of sublethal copper concentrations were assayed on Chlorella kess- leri, Coelastrum sphaericum and Scenedesmus acutus in autotrophic and mixotrophic media. The strains showed different susceptibility to copper in the bioassay, being C. sphaericum more resistant to metal exposure. Bleaching and lipid peroxidation were more pronounced in absence of organic carbon in C. kessleri and S. acutus, but remained unaffected in C. sphaericum. Acetate addition diminished free copper cation concentra- tion in growth media, increased growth rate in all cases and attenuated metal effect on bleaching and lipid peroxidation in C. kessleri and S. acutus. In C. sphaericum, the dele- terious effect on growth rate observed with the highest copper concentration might be due to copper-altered acetate assimilation.
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