Effects of Subacute and Subchronic Treatment of Synthetic Plant Growth Regulators on Liver Damage Serum Biomarkers Tissue Antioxidant Defense Systems and Lipid Peroxidation in Rats

Necati Ozok,Ismail Celik

doi:10.4172/2157-7609.1000124

Abstract

The present study was aimed to investigate the effects of some synthetic plant growth regulators; s-Naphthoxy acetic acid (s-NOA) and 4-Chlorophenoxy acetic acid (4-CPA) at 10 and 20 ppm dosages on liver damage, antioxidant defense system and lipid peroxidation in rats. Rats were divided into 12 groups as control, s-NOA and 4-CPA at 10 and 20 ppm dosages application groups. Serum marker enzymes and biochemical parameters were selected as biomarkers for liver damage. Antioxidant defense systems as SOD, GR, CAT, GSH-Px, GST and G6PD enzyme activities and GSH and MDA contents were determined in tissues as the indicators of oxidative stress. At the end of experiments, the statistically significant changes were observed in the levels of TPRO, TALB, TCHOL, TBIL, AST, ALT and LDH as liver damage serum biomarkers in rats exposed to 10 and 20 ppm of s-NOA and 4-CPA as compared with controls. MDA levels increased in all the tissues of rats exposed to s-NOA and 4-CPA dosages whereas the fluctuation in SOD, GR, CAT, GSH-Px, GST and G6PD enzyme activities and GSH levels were observed compared with controls. It could be concluded that the treatments of s-NOA and 4-CPA dosages may induce liver damage and oxidative stress.

Highlights

Many chemicals are currently used in agriculture, and plant growth regulators (PGRs) are among those widely used
The results of experiment showed that the treatment of rats with ß-Naphthoxy acetic acid (ß-NOA) and 4-chlorophenoxyacetic acid (4-CPA) caused changes in the activities and levels of serum parameters selected as biomarkers for liver damage (Table 1-2), in the concentration of MDA and GSH, and antioxidant enzymes such as CAT, G6DP, GST, GR, GSH-Px and SOD in erythrocyte, liver, brain, kidney and heart tissues in comparison to control rats (Table 3-12)
To find out the significance of biochemical changes in different tissues exposed to ß-NOA and 4-CPA for 25 and 50 days, the data have been subjected to ANOVA (One way) test

Summary

Introduction

Many chemicals are currently used in agriculture, and plant growth regulators (PGRs) are among those widely used. PGRs play important roles in many cellular processes including seed development, dormancy, germination, vegetative growth, and environmental stress responses [2]. PGRs play important roles in many cellular processes including promotes stem elongation, overcomes dormancy in seed and buds, involved in parthenocarpic fruit development, flowering, mobilization of food reserves in grass seed germination, juvenility and sex expression [3]. 2,4-Dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), and 4-chlorophenoxyacetic acid (4-CPA) are the chemicals belonging to this group. Among these agents, teratogenic, mutagenic, and carcinogenic effects of 2,4-D and 2,4,5-T are subjected to intense investigations [5]

Methods

Results

Discussion

Conclusion