Abstract
Objective:To study the effects of stromal interaction molecule 1 (STIM1) RNA interference on cell proliferation, apoptosis, invasion and migration ability in vitro in human laryngeal cancer cells Hep-2. Method:Lentivirus transfection was used to realize the STIM1 RNA interference in human laryngeal cancer cells Hep-2. STIM1 RNA interference was verified by real-time PCR and western blotting. The cell proliferation was analyzed by CCK-8 method, Transwell chamber and wound scratch assay were used to detect cell invasion and migration ability, respectively. The cell apoptosis rate was evaluated by flow cytometry. The apoptotic proteins expression levels of caspase-3, Bax, Bcl-2, VEGF and MMP9 were detected by western blotting. Result:The gene expression levels of STIM1 mRNA and protein in human laryngeal cancer cells Hep-2 were significantly decreased after interference lentivirus transfection. After the interference of STIM1, the cell proliferation, invasion and migratory ability of Hep-2 cells in vitro declined (P<0.05). The cell apoptosis rate significantly increased after STIM1 RNA interference, along with the caspase-3 and Bax protein expression levels up-regulation, and the Bcl-2 expression levels down-regulation. The decrease of cell invasion and migratory ability was related with the down-regulation of VEGF and MMP9 protein expression. Conclusion:RNA interference of STIM1 in human laryngeal cancer cells Hep-2 inhibits the cell proliferation, invasion and migration ability in vitro, and enhances the cell apoptosis rate of Hep-2 cells.
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Schedule a callMore From: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
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