Effects of spent mushroom substrate on the dissipation of polycyclic aromatic hydrocarbons in agricultural soil

Abstract

Spent mushroom substrate (SMS) is an agricultural waste with a high potential for polycyclic aromatic hydrocarbons (PAH) removal in aged contaminated soils. In this study, fresh and air-dried Pleurotus ostreatus, Pleurotus eryngii, and Auricularia auricular SMSs were used to remove PAHs in agricultural soil under 60-day incubation. The potential of SMS in PAH dissipation was studied by detecting the dissipation rate and the soil physicochemical index, enzyme activity, PAH-degradation bacterial biomass, and microbial diversity. Results showed that SMS significantly enhanced the dissipation of PAHs and fresh SMS had a better effect than air-dried SMS. The highest dissipation rate of 16 PAHs was 34.5%, which was observed in soil amended with fresh P. eryngii SMS, and the PAH dissipation rates with low and high molecular weights were 41.3% and 19.4%, respectively. By comparison, fresh P. eryngii SMS presented high nutrient contents, which promoted the development of PAH-degrading bacteria and changed the soil bacterial community involved in degradation, thereby promoting the PAH dissipation. The lignin-degrading enzymes in fresh SMS were abundant, and the laccase and manganese peroxidase activities in the treatment of fresh P. eryngii SMS was higher than those in other treatments. Fresh P. eryngii SMS improved the relative abundance of Microbacterium, Rhizobium, and Pseudomonas in soil, which were all related to PAH degradation. Consequently, adding fresh P. eryngii SMS was an effective method for remediating aged PAH-contaminated agricultural soils.