Effects of Sohamhyoong-Tang on Ovalbumin-Induced Allergic Reaction in BALB/c Mice.

So Hyun Jo,Yun Jung Lee,Min Cheol Park,Dae Gill Kang,Dae Ki Kim,Ho Sub Lee

doi:10.1155/2016/6286020

Abstract

IgE-mediated mast cell degranulation and excessive Th2 cells activation are major features of various allergic diseases. Sohamhyoong-tang has been reported to have anti-inflammatory and antibacterial effects. In this study, we investigated the inhibitory effect of Sohamhyoong-tang extract (SHHTE) on allergic symptoms and inflammatory responses in ovalbumin- (OVA-) sensitized BALB/c mice. The mice were sensitized with OVA and alum at 2-week intervals and then orally given SHHTE for 13 days followed by intradermal OVA injection. Administration of SHHTE significantly reduced edema formation and inflammatory-cell infiltration in ear tissues. Total and OVA-specific IgEs as well as proinflammatory cytokine TNF-α and Th2-associated cytokine IL-4 levels were lower in the SHHTE-treated group than in the vehicle. SHHTE treatment significantly suppressed both mRNA and protein levels of IL-4 and IL-5 in OVA-stimulated splenocytes. SHHTE decreased Th1 (IFN-γ) and Th17 (IL-17a) cytokine mRNA expression but increased Treg cytokines (IL-10 and TGF-β1). Moreover, SHHTE significantly inhibited degranulation of RBL-2H3 cell line in a dose-dependent manner. Thus, SHHTE efficiently inhibited the allergic symptoms in an OVA-sensitized mouse model and its action may correlate with the suppression of IgE production by increasing IL-10 and TGF-β1, which can limit the function of other T helper cells and prevent the release of inflammatory mediators from mast cells. These results suggest that SHHTE could be a therapeutic agent for treating various allergic diseases.

Highlights

Allergy is a concept that was initially introduced in 1906 by a pediatrician named Clemens, which meant harmful reactions of the host against foreign materials [1]
Histamine, TNF-α, and IL-1 released from mast cells by IgE-mediated inflammatory reactions and activation of Th2 cells, a type 2 helper T cell, which secrete a variety of proinflammatory cytokines such as IL-4, IL-5, and IL-13 play an important role and besides the roles of regulatory T cells and helper T cells such as Th1 and Th17 are involved in the regulation of allergic inflammatory reactions [4, 5]
IgEs are typically generated against allergens and mast cells and basophils in human and experimental animals have a strong affinity for the Fc portion of the IgE antibody

Summary

Introduction

Allergy is a concept that was initially introduced in 1906 by a pediatrician named Clemens, which meant harmful reactions of the host against foreign materials [1]. Allergy refers to an acute-type hypersensitivity reaction as an immune response that occurs within minutes after IgE antibodies attached to the surface of mast cells interact with antigens, which is the most common form of sensitization that occurs in 20% of the entire population [2, 3]. Histamine, TNF-α, and IL-1 released from mast cells by IgE-mediated inflammatory reactions and activation of Th2 cells, a type 2 helper T cell, which secrete a variety of proinflammatory cytokines such as IL-4, IL-5, and IL-13 play an important role and besides the roles of regulatory T cells and helper T cells such as Th1 and Th17 are involved in the regulation of allergic inflammatory reactions [4, 5]. In a study of the prevalence of CAM use, more than one-third of respondents reported using CAM [8]

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