Effects of siRNA against Survivin gene on the proliferation and apoptosis of human renal carcinoma cell line 786-O

Abstract

Objective To evaluate the effects of small interfering RNA (siRNA) against Survivin gene on the proliferation and apoptosis of human renal carcinoma cell line 786-O cells. Methods One pair of Survivin target sequence-specific siRNA was designed and synthesized, then siRNA/liposome complex was used to transfect the human renal carcinoma 786-O cells with increasing concentrations (10, 50, 100 nmol/L). The mRNA and protein expression of Survivin was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. The proliferation of 786-O cells was measured by MTT assay. The apoptosis of 786-O cells was examined by TUNEL assay. Results SiRNA-Survivin efficiently down-regulated the Survivin expression, inhibited the proliferation and induced the apoptosis in a dose dependent manner (for mRNA 88. 3% , 62.4% , 43. 8% , for protein 87. 7% , 62.4% , 46.5%, proliferation-inhibiting rate 11.6%, 41.2%, 57.5%, and apoptosis rate 14.2%, 29.4%, 38. 1% , respectively) (P<0.05). Conclusion siRNA against Survivin gene can inhibit the proliferation, respectively) (P<0.05). Conclusion siRNA against Survivin gene can inhibit the proliferation Key words: Renal cell carcinoma; Survivin gene; Small interfering RNA