Effects of trichostatin A on cell growth and histone deacetylase 1, matrix metalloproteinase 9 gene expression in renal carcinoma cells

Abstract

Objective To study the depressant effect on proliferation and the expression of histone deacetylase 1 (HDAC1) and matrix metalloproteinase 9 (MMP9) when the trichostatin A (TSA) was added to human renal carcinoma cell line 7860. Methods Four concentrations of TSA (100, 200, 400, 800 nmol/L) on7860 at different time durations of 12, 24, 36, 48 h were applied. The proliferation of 7860 was determined by methyl thiazolyl tetrazolium (MTT) method. The apoptosis was observed by AO/EB staining. The expression of HDAC1 and MMP9 in 7860 was examined by reverse transcription-polymerase chain reaction (RT-PCR) with TSA (400 nmol/L) at 24 h. Under the same testing condition, the protein expression level of HDAC1 and MMP9 in 7860 was detected by Western blotting, and the data were analyzed. Results The inhibition ratio was 32% , 45% , 58% , 62% in 7860 cells treated with TSA of 100, 200, 400, 800 nmol/L at 24 h. The apoptosis rate was increased after TSA treatment. The expression of HDAC1 and MMP9 mRNA in cancer cells was reduced after TSA treatment. The expression of HDAC1 protein was reduced by 18. 6%-87. 5% (P<0.01) and 21. 3%-91.4% (P<0.01), and that of MMP9 protein was decreased by 24. 6% -95. 7% (P<0.01) and 20. 8% -89. 2% (P<0.01) respectively as compared with control group and negative group. Conclusion TSA could inhibit the expression of HDAC1 and MMP9, and proliferation of 7860 simultaneously. Key words: Trichostatin A; HDAC1; MMP9; Renal cell carcinoma