Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721.

Jian Zhao,Jia-Le Song,Hewei Li,Chengqiang Wang,Zhewen Dong,Zixian Wang,Wanying Li,Huan Lan

doi:10.1155/2021/6095963

Jian Zhao, Jia-Le Song + Show 6 more

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https://doi.org/10.1155/2021/6095963

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Sinapic acid (Sa) is a small-molecule phenolic acid compound predominant in fruits, vegetables, and grains. This study investigated the antitumor effects of cisplatin (DDP) combined with Sa (Sa/DDP) on the hepatic cancer cells (HCC), HepG2 and SMMC-7721. The HepG2 and SMMC-7721 cells were treated with Sa or Sa/DDP, and the cell proliferation and cell cycle were detected using the MTT assay. The cell migration was detected using the transwell and scratch assays, while apoptosis and autophagy were detected using Hoechst, MDC, and Annexin V-FITC/PI staining. The protein expression was quantitated using the western blot. Sa/DDP was found to not only inhibit cancer cell proliferation and migration but also induce cell apoptosis. Simultaneously, the Sa/DDP combination was found to activate autophagy, and the HCQ autophagy inhibitor enhanced the apoptosis in the Sa/DDP-induced liver cancer cells. The combined use of Sa and DDP makes it an attractive adjuvant therapy strategy for tumors, establishing the prospect of phenolic acid compounds for the adjuvant treatment of hepatocellular carcinoma.

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Hepatocellular carcinoma (HCC) is one of the several malignancies posing a severe threat to human health and accounting for more than 85% of all primary liver cancer [1]
The antitumor effects of Sinapic acid (Sa) combined with DDP (Sa/DDP) on the HepG2 and SMMC-7721 cells and the impact of the Sa/DDP combination on autophagy and apoptosis are still unclear. is study aims to reveal the role of Sa/DDP in treating hepatic cancer and further explores its influence on autophagy and apoptosis
MO, USA). e cell cycle and apoptosis analysis kit (C1052), Hoechst staining kit (C0003), Annexin V-fluorescein-isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit (C1062), BeyoECL plus (P0018), BCA protein assay kit (P0012), HRP-labeled goat anti-rabbit IgG (H + L) (A0208) and rabbit anti-B-cell lymphoma-2(Bcl-2; AF0060), apoptosis regulator Bcl-2-like protein 4 (Bax; AF0057) polyclonal antibody and rabbit anti-poly ADP-ribose polymerase (PARP; AF1657), cleaved-PARP (c-PARP; AF1567), cysteine-requiring aspartate protease-3, active-caspase-3 (a-caspase-3; AF1150), and autophagy protein 5 (Atg5; AF2269) monoclonal antibodies were procured from Beyotime Institute of Biotechnology (Shanghai, China). e rabbit anti-β-actin (GB11001), sequestosome-1 (p62; GB11531) polyclonal, microtubuleassociated proteins 1A/1B light chain 3 (LC3I/II and GB11124), and Beclin-1 (GB3228-1) polyclonal antibody were procured from Servicebio Co., Ltd. (Wuhan, China)

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Introduction

Hepatocellular carcinoma (HCC) is one of the several malignancies posing a severe threat to human health and accounting for more than 85% of all primary liver cancer [1]. Combining the phenolic acid compounds with the standard chemotherapeutic drugs shows effective antitumor activity. E combination of rosmarinic acid with DDP has a sensitization effect on the DDP-resistant A549 lung cancer cells [12] Based on these reports, a combination of phenolic acid with the standard chemotherapy drugs is hypothesized to have an antitumor role. Is study aims to reveal the role of Sa/DDP in treating hepatic cancer and further explores its influence on autophagy and apoptosis.

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