Effects of short-chain fatty acids on oxidative stress and inflammation in glomerular mesangial cells induced by high glucose

Abstract

To investigate the effects of short-chain fatty acids(SCFAs)on oxidative stress and inflammation in cultured glomerular mesangial cells(GMCs)under high glucose. SV-40 MES 13 mouse GMCs were exposed to 30 mmol/L glucose, exogenous SCFAs or their specific G-protein coupled receptor 43(GPR43)agonist were used individually as an intervention. GPR43 expression was suppressed by transfection with GPR43-specifc siRNA. The oxidative stress-related factors reactive oxygen species and malondialdehyde were detected and the expression of GPR43, monocyte chemotactic protein 1(MCP-1), and interleukin-1β(IL-1β)were observed. GPR43 expression were significantly down-regulated, but reactive oxygen species and malondialdehyde production and MCP-1 and IL-1β releases were induced by high glucose(all P<0.05), treatment with SCFAs or GPR43 agonist reversed high glucose-induced oxidative stress and inflammation in a dose-dependent manner(all P<0.05). However, these SCFAs-mediated effects were blunted by siRNA-mediated knockdown GPR43(all P<0.05). SCFAs mitigates high glucose-induced oxidative stress and inflammatory injury in part via GPR43 signaling pathway, suggesting a likely mechanism for SCFAs-induced therapeutic effect in diabetic kidney disease. (Chin J Endocrinol Metab, 2018, 34: 239-245) Key words: Short-chain fatty acids; Oxidative stress; Inflammation; Diabetic kidney disease