Effect of high glucose-induced oxidative stress on proliferation of endothelial progenitor cells

Abstract

Objective To investigate the effect of high glucose-induced oxidative stress on proliferation of endothelial progenitor cells (EPCs) from rats.Methods Mononuclear cells were isolated from bone marrow in rats,and cultured for 3 days.According to the glucose concentrations after identification,two groups were set up:the control group (5.5 mmol/L) and the high glucose intervention group.The high glucose intervention group was divided into three subgroups:high glucose subgroup l (15 mmol/L),high glucose subgroup 2 (30 mmol/L),high glucose subgroup 3 (60 mmol/L).Anti-O2-,malondialdehyde ( MDA),glutathione ( GSH),and proliferation of EPCs were detected at 24,48,96 h respectively.Proliferation of EPCs was assayed again after the intervention of antioxidant therapy.Results The EPCs after culture for 3 days were confirmed.As compared with the control group at 48 h and 96 h,anti-O2-,proliferation of EPCs,and GSH in high glucose intervention group were significantly reduced ( P ＜ 0.05 ),and MDA significantly increased.As compared with those at 24 h,anti-O2-,proliferation of EPCs,and GSH were significantly reduced ( P ＜ 0.05 ),and MDA significantly increased in high glucose supgroup 2 and subgroup 3 at 48 and 96 h.As compared with the high glucose intervention group,proliferation of EPCs was significantly increased in the high glucose intervention + 2,2,6,6-four methyl pyridine oxide free radicals (TEMPO) group (P ＜ 0.05).Conclusion High glucose-induced oxidative stress was increased significantly in EPCs from rats,and proliferation of EPCs was significantly decreased.Proliferation of EPCs was significantly increased after treatment with anti-oxidants,suggesting that high glucose-induced oxidative stress may be one of important mechanisms influencing the proliferation of EPCs. Key words: Endothelial progenitor cells; High glucose; Oxidative stress; Malondialdehyde; Glutathione