Abstract
Abstract Sample solvents of weaker eluent strength than the mobile phase reduce band broadening induced by simultaneous mass and volume overload; but increase elution times of the sample components. As a result, better throughput is obtained when sample solubility allows for introduction of the sample in a small volume of the chromatographic mobile phase. However, under the constraint that a large injection volume must be employed to introduce the sample (frequently the case in reversed phase separations), the reduced bandwidths obtained in weaker sample solvents may offset the extended run time to provide better throughput.
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