Effects of salinity changes on larval survival, growth, and development of the edible sea cucumber, Holothuria leucospilota (Brandt, 1835) at two developmental stages

Abstract

For large-scale artificial breeding of sea cucumber (Holothuria leucospilota), it is necessary to understand how environmental parameters affect larval rearing. In this study, the effects of salinity changes on survival, growth, and development of the mid- and late-auricularia of H. leucospilota were estimated within a 7-day culture period. Salinity shock is usually accompanied by water replacement during hatching. To replicate the occurrence of salinity changes in actual larval production, 1/2 of the larval rearing water (initial salinity ∼31.6 ppt) was replaced daily by the experimental waters at different salinity levels (18, 22, 26, 30, 34, 38, and 42 ppt). On day 7, larval survival rates varied with salinity, while the highest survival rates (44.37–46.63% for mid-auricularia; 50.71–53.98% for late-auricularia) were observed in normal salinities (30 and 34 ppt) and the lowest survival rates (15.71–23.96% for mid-auricularia; 24.11–29.57% for late-auricularia) were observed in extreme salinities (18, 22, and 42 ppt). For any sampling event, the body length of mid-auricularia in normal salinities (30 and 34 ppt) were continued to increase during the 7-day culture period. The highest length of late-auricularia was reached on day 3 (880.01 ± 84.38 and 889.06 ± 112.11 µm, respectively), then decreased to 745.31 ± 127.64 and 742.94 ± 98.43 µm on day 7 due to metamorphosis. On day 7, the proportion of mid-auricularia larvae without development potential was the lowest (36.67%) at a salinity level of 34 ppt, and the proportions were increased with both decreasing/increasing salinities. However, this proportion was much lower for the late-auricularia at the same salinity. The decrease in salinity could promote metamorphosis of the late-auricularia, and the highest proportion of doliolaria (24.32%) was observed at salinity level of 26 ppt on day 7. In extreme salinities (18, 22, and 42 ppt), the hyaline spheres (indication of larval competence) in late-auricularia were less obvious than those in other salinities. The ability in resisting salinity shock was increased with larval development, where late-auricularia had better tolerance to salinity shock than mid-auricularia, whereas larvae at both stages were more tolerant to hypertonic than hypotonic conditions. Based on our results, we propose to rear the mid- and late-auricularia at a comparatively high level of salinity (e.g., ∼31 ppt) to promote their survival and performance, while to decrease the salinity (e.g., ∼26 ppt) in the last period of late-auricularia to increase their competence to metamorphose into doliolaria.