Effects of rumen-protected choline supplementation in Holstein dairy cows during electric heat blanket-induced heat stress

Abstract

Dairy cows experiencing heat stress (HS) attempt to thermoregulate through multiple mechanisms, such as reducing feed intake and milk production and altering blood flow to increase heat dissipation. Effects of choline on energy metabolism and immune function may yield it a viable nutritional intervention to mitigate negative effects of HS. The primary objective of this experiment was to determine if supplementation of rumen-protected choline during, or before and during, an increased heat load would ameliorate the negative effects of HS on production and immune status. Heat stress was induced via an electric heat blanket model with a 3-d baseline period and 7-d HS period for all cows. Multiparous mid-lactation (208 ± 31 days in milk) Holstein cows were fed the same basal herd diet, blocked by pre-experiment milk yield, and randomly assigned to receive one of the following: (1) no rumen-protected (RP) choline (n = 7); (2) RP choline (60 g/d) via top-dress during the HS period (n = 8); or (3) RP choline (60 g/d) via top-dress during the baseline and HS periods (n = 8). Imposing HS via electric heat blanket raised respiration rate with all cows surpassing the HS threshold of 60 breaths/min. The increase in respiration rate tended to be ameliorated with either schedule of RP choline supplementation. Milk yield tended to increase when RP choline was supplemented in both the baseline period and during HS. Supplementation of RP choline tended to reduce blood fatty acid and triglyceride and tended to increase the revised quantitative insulin sensitivity check index. The role of RP choline supplementation to partially ameliorate the effects of HS should be further explored as a potential nutritional strategy to mitigate the negative consequences of HS on health and production.