Effects of rosiglitazone on expression of type I collagen and type III collagen in hepatic stellate cells

Abstract

Objective To investigate the effects of rosiglitazone,a ligand of peroxisome proliferators activated receptor gamma (PPARγ),on the expression of type Ⅰ collagen (COL Ⅰ) and type Ⅲ collagen (COL Ⅲ) in hepatic stellate cells (HSCs).Methods HSCs were cultured in medium containing different concentrations of rosiglitazone (5,10,15,20 μmol/L respectively),and harvested at 24 h.The expression of COL Ⅰ and COLⅢ was detected by using reverse transcription polymerase chain reaction.Results The COL Ⅰ expression of HSCs cultured with different concentrations of rosiglitazone (5,10,15,20 μmoL/L) was 2.9755 ± 0.0853,1.9866 ± 0.0938,1.4935 ± 0.1078 and 0.9603 ± 0.1365 respectively,and it in control group was 3.4802 ±0.1331.The COLⅢ gene expression in HSCs cultured with different concentrations of rosiglitazone (5,10,15,20 μ mol/L) was 3.6207 ± 0.0461,2.6150 ±0.0793,2.0051 ± 0.0830 and 1.4175 ± 0.0862 respectively,and it in control group was 4.2714 ±0.4652.Conclusion PPARγ ligand rosiglitazone may down-regulate the gene expression of COL Ⅰ and COLll in HSCs dose-dependently to some extent. Key words: Hepatic stellate cell; Peroxisome proliferators activated receptor gamma; Rosiglitazone; Type Ⅰ collagen ; Type Ⅲ collagen