Effects of ROS on hypoxia- and cobalt-induced HIF-1 binding and erythropoietin expression in HEP3B: [formula omitted] William G. Willmore, and H. Franklin Bunn. Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115

Abstract

The ING4 tumor suppressor plays a significant role in various cancer-related cellular processes. AUF1 affects the stability and/or translation of multiple mRNAs via binding to an AU-rich element in the 3′-untranslated regions. In this study, we identify AUF1 as a novel and direct binding partner of ING4. mRNP immunoprecipitation assays indicated that ING4, AUF1 and MYC mRNA present in the same mRNP complex. ING4 suppressed MYC protein expression without altering MYC mRNA levels, and abolished the cell proliferation induced by AUF1 in K562 cells. These results suggest that ING4 may regulate MYC translation by its association with AUF1.