Abstract
Abstract:Transformed root lines of Datura stramonium were established following infection of aseptic stem segments with Agrobacterium rhizogenes strain A4. These root lines were examined for their alkaloid productivity in relation to growth rate and the presence in the nuclear genome of rol(A, B and C) and tms genes. The amount of polypeptides coded by rolB and rolC genes, and the transcript levels of rolA, rolB and rolC genes were also investigated. Southern blot analysis using [32P]‐la‐belled fragments of the T‐DNA as probes, revealed the lack of tms genes and the presence of the three rol genes in the genome of the all root lines. The growth rates of these transformed root lines were not always correlated with their hyoscyamine biosynthesis ability. However, all transformed root lines, showed a positive correlation between hyoscyamine production and the level of polypeptide coded by rolC gene.
Published Version
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