Abstract
BackgroundNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. This study aims to consider effects of resveratrol, exercise and their combination on Farnesoid X receptor (Fxr), the liver X receptor (Lxr) and Sirtuin 1 (Sirt 1) genes expression in the liver of elderly rats with NAFLD.MethodsRats with NAFLD were randomly divided into seven groups including patient, saline, resveratrol (RSV), interval exercise, continuous exercise, interval exercise + RSV and continuous exercise + RSV. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the liver tissue were measured using specific ELISA kits. A TUNEL assay kit was used for the assessment of hepatic cells apoptosis. Lipid profiles were considered by measuring the serum triglyceride, cholesterol, LDL, and HDL. Expression of Sirt1, Lxr and Fxr genes was considered using RT-PCR.ResultsResveratrol administration alone or combined with exercise training significantly improved the expression of Sirt1, Lxr and Fxr genes (p < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased (p < 0.05).DiscussionAlthough resveratrol alone improves the expression of Sirt1, Lxr and Fxr, as well as liver function, combined therapy with exercise training is more effective to improve NAFLD.
Highlights
Non-alcoholic fatty liver disease (NAFLD) is a common type of chronic hepatic disease that affects 25–30% of people in the world (Li et al, 2015)
We evaluated the positive effect of resveratrol supplementation, exercise training and their combination on Sirtuin 1 (Sirt1), liver X receptor (Lxr) and Farnesoid X receptor (Fxr) gene expression, hepatic specific enzymes, lipid profiles and hepatic cell apoptosis in old rats with moderate NAFLD
We have found that moderate NAFLD is associated with reduced expression of Lxr, Fxr, and Sirt1 as well as increased levels of hepatic injury biomarkers
Summary
Non-alcoholic fatty liver disease (NAFLD) is a common type of chronic hepatic disease that affects 25–30% of people in the world (Li et al, 2015) It is associated with a significant accumulation of lipids in the liver and severe abnormalities in liver enzymes (Abd El-Kader & El-Den Ashmawy, 2015). This study aims to consider effects of resveratrol, exercise and their combination on Farnesoid X receptor (Fxr), the liver X receptor (Lxr) and Sirtuin 1 (Sirt 1) genes expression in the liver of elderly rats with NAFLD. Resveratrol administration alone or combined with exercise training significantly improved the expression of Sirt, Lxr and Fxr genes (p < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased (p < 0.05). Resveratrol alone improves the expression of Sirt, Lxr and Fxr, as well as liver function, combined therapy with exercise training is more effective to improve NAFLD
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