Abstract

Performance of anaerobic sludge and microbial social behaviors in an expanded granular sludge bed (EGSB) were evaluated by increasing reflux ratio from 50% to 500% stage by stage, with a constant influent chemical oxygen demand (COD) of 5500 mg/L at hydraulic retention time 12 h. The results indicated that the reflux ratio of 100% − 200% was more favorable for the EGSB with a methane production of 2.4 m3/m3·d. It was found that acyl-homoserine lactones (AHLs)-mediated quorum sensing (QS) could balance various microbial populations in the anaerobic digestion process. C4-HSL and C8-HSL were identified as the specific AHLs in enhancing granulation of anaerobic sludge by stimulating protein secretion into extracellular polymeric substances (EPS). 3-oxo-C6-HSL and 3-oxo-C14-HSL were verified for the enhancement of methanogenesis. The present study showed a novel perspective on the performance of EGSB with reflux ratios based on the AHLs-mediated QS.

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