Abstract
ABSTRACT In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes. Preadipocytes from healthy one-day-old calves were collected, isolated, and cultured in vitro. The expression pattern of the PSMA1 gene was explored during the differentiation of bovine preadipocytes firstly. Then, the expression of the PSMA1 gene was inhibited by transfection of a chemically synthesized small interfering RNA (siRNA) before differentiation. After induction [...]
Highlights
As the main organ of energy storage in animal bodies, adipose tissue plays an important role in regulating metabolism
Significantly fewer lipid droplets were formed by adipocytes transfected with proteasomal subunit α-1 (PSMA1)-small interfering RNA (siRNA) than by the negative control group
Since the expression level of PSMA1 was the highest at four days after induction under normal culture conditions, the key regulatory factors (Pref-1, PPARγ, C/EBPα, and lipoprotein lipase (LPL)) of preadipocyte differentiation and lipid deposition in preadipocyte that was transfected with PSMA1-siRNA changed
Summary
As the main organ of energy storage in animal bodies, adipose tissue plays an important role in regulating metabolism. Fat (or fatty acid) content has an important influence on meat quality and flavor (Lee et al, 2010; Corino et al, 2008). PSMA1 is an important component of the proteasome and plays an important role in regulating the physiological state of the body and the occurrence of cancer (Lee and Ryu, 2017; Wang et al, 2018). Recent studies have found that PSMA1 is differentially expressed during fatty acid metabolism and lipid production
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