Abstract
METHODS: W ith Research Ethics Committee approval, to evaluate whether continuous infusion of propofol (Diprivan, AstraZeneca, UK) could protect the liver from warm IRI in vivo, livers of male Sprague-Dawley rats were subjected to 60 minutes of partial normothermic ischemia allowing perfusion to right and caudate lobes and subsequent 45 minutes of reperfusion using a microsurgical clip after 30 minutes of stabilization. Either propofol (Propofol group, n = 11, 10 mg/kg/h) or saline (Control group, n = 11, same volume) was continuously administered during the stabilization period and throughout the experiment via the tail vein. At the end of reperfusion blood and liver tissue samples for SGOT and SGPT, lipid peroxidation assay, and histological examination for a morphometric tissue injury scoring system were taken. Additional eight rats were sacrificed for obtaining normal values of SGOT and SGPT. Data were analyzed using Mann-W hitney-U test and ANOVA with post hoc Scheffe test, P < 0.05 being significant.
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