Abstract
Lactococcus lactis JCM5805 plays an important role in tilapia farming to promote individual growth, immunity and disease resistance. This study applied gas chromatography/mass spectrometry (GC/MS) and high-throughput sequencing in characterizing microbial composition as well as metabolite profiles within tilapia gut fed with control and JCM5805-suppmeneted diets. Our results showed that JCM5805 administration altered the composition of host gut microbiota. The significant metabolic pathways in terms of microbial function between two groups included carbohydrate metabolism, nucleic acid metabolism, energy metabolism, and translation. In addition, the functions of differential metabolites between two groups were mainly related to metabolism, ABC transporter, metabolism of methionine and cysteine, amino acid biosynthesis, arachidonic acid metabolism, protein digestion/absorption, and asthma. There was significant interdependence between metabolites and microorganisms. Besides, JCM5805 distribution displayed significantly positive correlation with the up-regulation of metabolite L-cystine (CC > 0.90, CCP <0.01), which was involved in body protein metabolism. Findings in this study helped to understand the relationships of gut microbiota and metabolites in gut contents of fish administered with probiotics, and highlighted the possible material basis and mechanism of JCM5805 in affecting host growth and immunity.
Published Version
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