Effects of Priming Techniques on Seed Germination and Early Growth Characteristics of Bromus tomentellus L. and Bromus inermis L.

Ali Tavili,Salman Zare,Seyed Amir Moosavi,Abdolghader Enayati

doi:10.15835/nsb213613

Abstract

Seed priming is known as a seed treatment which improves seed performance under environmental conditions. Objective of this study was to evaluate the effects of different priming treatments on seed germination behaviour of two genotypes of Bromus under laboratory conditions to find out the most effective priming treatment. Seeds were treated with the following seed-soaking media: (i) unsoaked seed (control); (ii) hydropriming with distilled water for 4, 8, 12 and 16 hours, (iii) osmopriming treatments with PEG 6000 for Bromus tomentellus were -0.6, -0.8, -1.0 and -1.2 MPa osmotic potentials with duration of 12, 24 and 36 hours and for Bromus inermis osmoticpotentials were the same as B. tomentellus but duration of priming treatments was soaking seeds for 12, 24, 36 and 48 hours. Seeds of both genotypes were placed in liquid priming media at 25oC. Priming treatments significantly affected germination vigour of both genotypes. The response of both genotypes to different priming techniques approximately was similar. Osmopriming treatment (-0.6 MPa and 12 h) increased final germination percentage of B. tomentellus. Priming treatments increased coleoptile length significantly comparing to control but hydropriming for 4 h exhibited longer coleoptile than others. Higher vigour index was observed in hydropriming for 12 h but this difference was not significant with osmoprime treatment (12 h-0.6 Mpa). B. inermis produced more germinated seeds and vigourous seedlings than B. tomentellus but germination rate was higher in B. tomentellus.

Highlights

Seed priming is known as seed treatment wich improves seed performance under environmental conditions
The previous studies indicate that some benefits are associated with pre-sowing treatments for seed vigour enhancement, but there is dearth of information about the germination performance of primed seeds of Bromus spp
Seeds were treated with the following seed-soaking media: (i) unsoaked seed; (ii) hydropriming with distilled water for 4, 8, 12 and 16 hour; (iii) osmopriming treatments with PEG 6000 for B. tomentellus were -0.6, -0.8, -1.0 and -1.2 MPa osmotic potentials with duration of 12, 24 and 36 hours while for B. inermis osmotic potentials were the same as B. tomentellus but duration of priming treatments was soaking seeds for 12, 24, 36 and 48 hours

Summary

Introduction

Seed priming is known as seed treatment wich improves seed performance under environmental conditions. Earlier works showed that the success of seed priming is influenced by the complex interaction of factors including plant species, water potentiality of the priming agent, duration of priming, temperature, seed vigour and dehydration, and storage conditions of the primed seed (Parera and Cantliffe, 1994). The previous studies indicate that some benefits are associated with pre-sowing treatments for seed vigour enhancement, but there is dearth of information about the germination performance of primed seeds of Bromus spp. The present study was carried out with the objective of evaluating the effects of different priming treatments on seed germination behaviour of two genotypes of Bromus under laboratory conditions to find out the most effective priming treatment

Methods

Results

Conclusion