Effects of pregnancy vitamin D status on adipose tissue development and inflammation in lean, male adult mice offspring (1037.4)

Abstract

The bioactive form of vitamin D, 1,25‐dihydroxyvitamin D, and its receptor have been identified for a role in preadipocyte differentiation to adipocytes through the modulation of adipogenic transcript factor genes. We sought to determine if maternal vitamin D status influences adipose tissue development or inflammation in adult offspring. Female C57BL/6J mice were randomized to either a vitamin D deficient diet or a control diet (AIN‐93G) for 21 days prior to mating and maintained on this diet throughout pregnancy until postnatal (PN) day 7, at which point both dietary groups were switched to the control diet. At PN day 75, perigonadal (PGAT) and subcutaneous (SQAT) adipose tissue were collected from adult male offspring for general histology and mRNA expression of adipogenic genes. Serum was also collected. There were no differences between groups in whole body weight, fat pad weights, or adipocyte size or number. Mice born to vitamin D deficient dams had elevated serum concentrations of the pro‐inflammatory markers interleukin‐2 and resistin. Maternal vitamin D status during pregnancy does not appear to influence the development of adipose in lean male offspring maintained on a standard diet; however, increases in circulating resistin, an adipose‐derived protein, suggests an adipocyte response.Grant Funding Source: Supported by: UM Research Investment Fund and SIRC Research Grant