Effects of Polyploidization on Locomotor and Flight Activity, and Body Size of Males in Japanese Bumblebee, Bombus ignitus

Abstract

Polyploid animals are seen in the wild in only a few groups such as planaria, weevils, ants, wasps, and bees. The sex of bumblebees is determined by the single-locus complementary sex determination (sl-CSD) system resulting in females emerging from fertilized eggs and males (normal males, haploid) emerging from unfertilized eggs. However, diploid males (polyploids) emerged from fertilized eggs when the sex-determining locus become homozygous caused by inbreeding due to population contraction. To examine the effects of polyploidization on behavioral characteristics in bumblebee males, Bombus ignitus, haploid and diploid males were compared in locomotor activity, ease of staying inside the hive, and flight activity in a laboratory experiment. The results showed that diploid males had higher locomotor activity levels, were less likely to stay inside the hive, and had lower flight activity levels than haploid males. The reduced flight activity levels may lead to lower mating rates in the field. Furthermore, the body size of diploid males was smaller than that of haploid males. Diploid males of bumblebees have a low reproductive and produce diploid sperms to emerge sterile triploids, thus creating a considerable genetic load on the population. The behavioral and morphological characteristics of diploid males may unintentionally serve as a mechanism to prevent the emergence of sterile triploids.