Effects of polyisoprenoids from Avicennia lanata and Avicennia alba leaves on the gene expression of PI3K, Akt1, mTOR, P53, and EGFR in human colorectal adenocarcinoma WiDr cells

Taufiq Qurrohman,Etti Sartina Siregar,Sumaiyah Sumaiyah,Arif Nuryawan,Mohammad Basyuni,Poppy Anjelisa Zaitun Hasibuan

doi:10.12688/f1000research.22021.3

Taufiq Qurrohman, Etti Sartina Siregar + Show 4 more

Open Access

https://doi.org/10.12688/f1000research.22021.3

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Journal: F1000Research	Publication Date: Oct 26, 2020
License type: CC BY 4.0

Affiliation: University of North Sumatra

Abstract

Background:Mangrove plants produce polyisoprenoid compounds. Polyisoprenoids have been proven to have anticancer properties. This study investigated the inhibitory activity of polyisoprenoids derived from the leaves of mangrove plantsAvicennia albaandAvicennia lanataregarding the expression of PI3K, Akt1, mTOR, P53, and EGFR genes against human colorectal adenocarcinoma WiDr cells.Methods:Anticancer activity was tested through the MTT assay method performed on WiDr cells. The cell cycle and apoptosis were analysed by flow cytometry and double staining. Gene expression of PI3K, Akt1, mTOR, P53, and EGFR was observed using the RT-PCR method.Results:Cytotoxic activity against WiDr cells showed that the IC50 forA. albaandA. lanatawas 258.14 ug/mL and 243.32 ug/mL, respectively. This observation indicated the possibility to develop moderate anticancer agents. The cell cycle showed that inhibition ofA. albaandA. lanataoccurred in the late phase of apoptosis S (10.60 and 10.51%) and G2-M1 (22.05 and 23.84%), which was higher than negative and positive control cells. Furthermore, the polyisoprenoids derived fromA. albaandA. lanataleaves exhibited anticancer activity in WiDr cells through the downregulated gene expression of PI3K, Akt1, mTOR, and EGFR as well as the upregulated gene expression of P53.Conclusion:This study demonstrated that polyisoprenoids obtained fromA. albaandA. lanataleaves are promising chemopreventive agents for colon cancer.

Highlights

This study demonstrated that polyisoprenoids obtained from A. alba and A. lanata leaves are promising chemopreventive agents for colon cancer
Forward and reverse primers have been designed to trigger selective reverse transcription-polymerase chain reaction (RT-PCR) reactions for plasmid mRNAs11.the present study aims to investigate the inhibitory activity of polyisoprenoids obtained from the leaves of mangrove plants A. alba and A. lanata concerning the expression of PI3K, Akt[1], mTOR, P53, and EGFR genes in human colorectal adenocarcinoma WiDr cells
The cytotoxic test material was derived from polyisoprenoids of mangrove leaves (PAA and PAL) against colon cancer cells (WiDr) with a concentration series of 1000, 500, 250, 125, and 62.5 μg/mL

Summary

Introduction

This study investigated the inhibitory activity of polyisoprenoids derived from the leaves of mangrove plants Avicennia alba and Avicennia lanata regarding the expression of PI3K, Akt[1], mTOR, P53, and EGFR genes against human colorectal adenocarcinoma WiDr cells. Gene expression of PI3K, Akt[1], mTOR, P53, and EGFR was observed using the RT-PCR method. Results: Cytotoxic activity against WiDr cells showed that the IC50 for A. alba and A. lanata was 258.14 ug/mL and 243.32 ug/mL, respectively. This observation indicated the possibility to develop moderate anticancer agents. The cell cycle showed that inhibition of A. alba and A. lanata occurred in the late phase of apoptosis S (10.60 and version 4 (revision)

Methods

Results

Conclusion