Abstract
The cell-type composition of the initial cell population from protease-dispersed neonatal rat heart tissue has been evaluated using time lapse photography and identification of cell type-specific functions. The effects of two commonly employed plating densities on growth and cell division of the two major cell types were examined. Total protein synthesis rates were not affected by plating density but did change with age in culture. Maximum protein synthesis rates were observed during the period of maximum cell division and cell growth (increase in total cell protein), which was from 24 h in culture to the 4th d in culture. After 6 d in culture, synthesis rates for total proteins remained constant for at least 2 wk. Sizing of cells by Coulter counter analysis indicated that essentially all the cells were increasing in size with age in culture.
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