Effects of platelets rich fibrin and bone marrow on the healing of distal radial fracture in local dogs: Comparative study

Moyaser Thanoon,M.J Eesa,E.R Abed

doi:10.33899/ijvs.2019.163169

Abstract

The aim of this study was to evaluate the effects of platelets rich fibrin and bone marrow on distal radial fracture healing in local breed dogs. Twenty four adult animals (males and females), mean aged 2.6 ±0.15 years, and the mean body weight 24.58 ±1.07 kg were used. The experimental animals were randomly divided into three equal groups. First group (Control group), transverse fracture was induced in the distal portion of radial bone and immobilized by Plaster of Paris, the fracture line didn't treated with any material. Second group (platelets rich fibrin group), the fracture line was surrounded by the platelets rich fibrin. Third group (bone marrow group), the fracture line was surrounded by autologous bone marrow. The radiographic results confirmed that the fracture healing was faster and superior in third group compared with first and second groups. The hematological results showed an increasing in concentration rates of alkaline phosphatase enzyme and calcium at the weeks that follows surgical operation. Depending on the serial rates of alkaline phosphatase, the third group and the second group are the better fractured bone healing than the first group. In conclusion, the results revealed that, the using of bone marrow and platelets rich fibrin improved and enhanced the healing of distal radial fracture.

Highlights

The distal radial fracture is considered third most common fracture in dogs
In the first group (C), the fracture line didn't treat with any substance; while in the second group (PRF) the fracture line was surrounded by the PRF, which prepared just before beginning of operation, by drawing a whole blood 10cc from cephalic vein collected in test tube without anticoagulants centrifuged at 3000 rpm for 10 minutes, after centrifugation we will obtained a tube having three layers; the top one it is poor platelets plasma, the middle one it is the platelets rich fibrin, and at the bottom of the tube it’s a red blood cells (RBC) layer
These results coincides by McCartney et al and Manchi et al [4,16], whom said that, the lameness is clinically cleared when the distal radial fracture accompanied by ulnar fracture and is less when only distal radial fracture without ulnar fracture

Summary

Introduction

The distal radial fracture is considered third most common fracture in dogs. They represent approximately 17% of the all canine fractures and about 85% of the all radial fractures [1]. The traumatic injuries which result from traffic accidents, fighting, fall, gunshot and explosive projectiles are the main causes of distal radial fractures [3]. Reduce blood supply of distal radius region with little of soft tissues enveloping the fractured site, considered as the most important factors contributes for delayed or incomplete healing of distal radial fracture [2]. The delay-union, mal-union or even non-union healing is common in distal radial fracture especially in toy breeds dogs [4]

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