Abstract
To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine.
Highlights
The skin healing is the aim of studies and researches due to its clinical, scientific and economic interest, especially due to the great frequency of wounds caused by injury in horses
The objective of this study was to establish a protocol of the Platelet-rich plasma (PRP) gel from horse whole blood, growth factors, and autogenous thrombin, and to do the first comments with the application of the PRP gel in surgical wound healing in sequentially sampled biopsies in horses
No clinical complications were observed in the surgical wound healing in any horse
Summary
The skin healing is the aim of studies and researches due to its clinical, scientific and economic interest, especially due to the great frequency of wounds caused by injury in horses. In equine the healing of surgical or traumatic wounds delay and many times the cost limits the success of the healed wound. For this reasons, successful cutaneous wound healing needs combination drug therapy. A rich source of the complex group of growth factors (GF’s) essential to natural wound repair is the platelet[3]. The platelets act in the haemostasis; wound healing and reepithelialization liberating diverse GF’s that stimulate the angiogenesis, promoting growth and vascular fibroblast proliferation that in turn provide an increase in the collagen synthesis[4,5]. Platelet-rich plasma (PRP) is 100% biocompatible and safe. It poses absolutely no infectious risk to the patient because it is made from the patient’s own plasma
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