Effects of pioglitazone on AdipoR1 expressions in THP-1-derived macrophages

Abstract

Objective To study the effect of pioglitazone(PIO)on Adipo R1 and cholesterol ester(CE)in foam cells derived from THP- 1- derived macrophages. Methods THP- 1- derived macrophages were incubated with increasing concentrations of PIO for 24 hours. After co-cultured with low density lipoprotein (LDL), the accumulation of cholesterol in macrophages was measured by fluorescence spectrophotometric method. The lipid peroxide within cells was detected by TBARS method, the foam cells were observed by oil red staining. AdipoR1 levels were determined by Western blot. Results Compared with the ox-LDL group (0μmol/L),oil red O- positive cells of the PIO protective groups were greatly reduced.TC,CE,MDA of the PIO protective groups were also obviously decreased.TC(53.6±1.2)μg/mg,CE(30.2±3.6)μg/mg, MDA(3.42 ±0.06)μg/mg of 5μmol/L PIO group were lower than those of 0μmol/L PIO group[(98.2±3.5),(65.5±6.5),(8.50±1.21)]μg/mg(P< 0.05).TC(25.6±1.8)μg/mg,CE(22.5±4.5)μg/mg,MDA(1.90±0.42)μg/mg of 50μmol/L PIO group.TC(16.8±2.2)μg/mg,CE(5.9±1.4)μg/mg,MDA(0.65±0.05)μg/mg of 100μmol/L PIO group. Concomitantly, PIO significantly increased AdipoR1 protein expresion, AdipoR1 of 5μmol/L PIO group (0.06±0.05) was higher than that of 0μmol/L PIO group(0.03±0.07). AdipoR1 of 50μmol/L PIO group(0.11 ±0.07) was higher than that of 5μmol/L PIO group(0.06±0.05). AdipoR1 of 100μmol/L PIO group(0.40±0.05) was obviously higher than that of 50μmol/L PIO group(0.11±0.07). Conclusion PIO inhibited THP-1-derived formation by up-regulation the expression of AdipoR1, which may play an important role in the development and progression of atherosclerosis. Key words: Pioglitazone; Macrophages; AdipoR1