Abstract
Medicinal plants are one of the most vital natural resources, but many of them are currently endangered due to habitat loss. Consequently, it is critical to emphasize the importance of using micropropagation techniques for mass propagation of plantlets on a commercial scale, in addition to germplasm conservation and distribution. Nodal explants and shoot tips were expunged from 15 days of the explant by aseptic seedlings, an effective, quick, and better in vitro plant regeneration procedure for Vitex negundo L. has been developed. The recent study was considered to develop an in vitro procedure for the regeneration of V. negundo L., a traditional medicinal plant. Nodal segments and shoot tips were cultivated on MS medium enhanced with numerous plant growth regulators. For multiple shoots and root regeneration, various cytokinins were examined. 6-benzyl-aminopurin (BAP), kinetin (Kin), and 1H-indole-3-butanoic acid (IBA) were all tested as a supplement to Murashige and Skoog (MS) medium including auxin phytohormone, such as Indole acetic acid (IAA) and 1-naphthaleneacetic acid (NAA). The furthermost effective surface sterilization treatment for explants of V. negundo has been found 0.1% HgCl2 for 8 minutes. In all treatments, multiple shoots were collected from shoot tips and nodal segments. In MS media added with 2.0mg/l BAP, the most shoots were seen in V. negundo. Furthermore, V. negundo regeneration shoots rooted effectively in half MS containing 1.0 mg/l IBA. Finally, proliferated plantlets were effectively adapted in soil, where they grew normally without morphological anomalies and had a survival rate of 92 percent.
Highlights
Tissue culture of the plant is an in vitro approach that has appeared as a promising means of propagation and enhancing genomic variety for plant enhancement, and it is used in a variety of industries including agriculture, horticulture, and forestry (Nitish and Reddy, 2011)
3.1 Standardization of explant (Vitex negundo L.) surface sterilization After cutting into suitable size the nodal segments and shoot tips of V. negundo were washed with the continuous flowing of tap water for 20m and treated with antiseptic (4-5 drops liquid soap along with 2-3 drops of Tween-20) and again washed with ddH2O
After one week of sterilization and inoculation it was detected that, when the experimented plant parts were treated with 0.1% HgCl2 for 8 minutes, 95% of V. negundo L. explants were found contamination-free and healthy
Summary
Tissue culture of the plant is an in vitro approach that has appeared as a promising means of propagation and enhancing genomic variety for plant enhancement, and it is used in a variety of industries including agriculture, horticulture, and forestry (Nitish and Reddy, 2011). In 1902, a German scientist named Haberlandt got promising results on in-vitro plant growth, making him the first to put plant tissue culture into practice. The current study deals with the comparative performances of a variety of cytokinins including IBA, BAP, and Kinwere all tested singly or in numerous mixtures to MS medium with the various combination of NAA, and IAA on the in vitro revival of root and shoot by using shoot tips and nodal segmentsof V. negundo by successful restoration plants into field environments
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