Effects of Photobiomodulation on High Glucose Induced Oxidative Stress in Human Embryonic Skin Fibroblasts

Abstract

In this study, we explored the effects of photobiomodulation (PBM) on the functional damage of human embryonic skin fibroblasts (CCC-ESFs) in a high glucose environment, as well as the effects of PBM on tissue repair, to provide a theoretical basis for application and promotion of dual-wavelength laser therapy in clinical practices. CCC-ESFs were cultured in vitro for 2 d in a high glucose medium, and then irradiated with a 635/808 nm dual-wavelength semiconductor laser at a dose of 20 mW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , 0-24 J/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . Cell proliferation, reactive oxygen species (ROS) production, antioxidant enzyme activity (CAT, SOD), lipid oxidization capacity (MDA), cell growth factors, and the expression of matrix metalloproteinases (MMPs) and matrix metalloproteinase inhibitors (TIMPs) were assessed. We found that PBM can enhance the proliferation of fibroblasts, improve the inhibitory effects of the high glucose environment on cell proliferation, increase intracellular ROS production, enhance the activity of antioxidant enzymes, and stimulate cytokine secretion. Furthermore, PBM was found to inhibit the expression of MMPs, increase the expression of TIMPs, reverse the overexpression of MMPs/TIMPs caused by high glucose, and promote wound healing.