Effects of genistein on the mRNA expressions of collagen, matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase in human embryonic skin fibroblasts

Abstract

Objective To investigate the effects of genistein on the mRNA expressions of collagen (Col), matrix metalloproteinase (MMP ) and tissue inhibitor of matrix metalloproteinase (TIMP) in human embryonic skin fibroblasts (CCC-ESF-1). Methods The cultured CCC-ESF-1cells were divided into a black control group, an estradiol group and genistein groups of different doses. The mRNA expressions of ColⅠ , Col Ⅲ, MMP-1, TIMP-1 and TIMP-2 were detected by RT-PCR. Results Compared with the black group, estradiol and medium dose of genistein (0.451 ± 0.037, 0.446 ± 0.047 vs. 0.385 ± 0.061, all P<0.05) could promote the proliferation of the CCC-ESF-1 cells, estradiol and medium dose of genistein could up-regulate the mRNA expressions of ColⅠ (0.960 ± 0.012, 0.929 ± 0.015 vs. 0.812 ± 0.014, all P<0.01), Col Ⅲ (0.892 ± 0.009, 0.824 ± 0.022 vs. 0.768 ± 0.025, all P<0.01), TIMP-1 (0.841 ± 0.023, 0.838 ± 0.053 vs. 0.751 ± 0.027, all P<0.01) and TIMP-2 (0.456 ± 0.017, 0.448 ± 0.036 vs. 0.381 ± 0.029, all P<0.01), and down-regulate MMP-1 mRNA expression (0.398 ± 0.043, 0.402 ± 0.044 vs. 0.525 ± 0.006, all P<0.01). Conclusions Genistein could promote the proliferation of the CCC-ESF-1 cells, and that may be related with up-regulating the mRNA expressions of ColⅠ,Col Ⅲ, MMP-1, TIMP-1and down-regulating MMP-1 mRNA expression. Key words: Fibroblasts; Genistein; Collagen; Matrix metalloproteinase 1; Matrix metalloproteinase inhibitors