Effects of Phenolic Inhibitors on Growth and Metabolism of Glucose-Ul- 14 C in Paul's Scarlet Rose Cell-Suspension Cultures

Abstract

ABSTRACT Paul's Scarlet rose cell-suspension cultures were incubated in varying concentrations of the following phenolic inhibitors; chlorogenic acid, cinnamic acid, p-coumaric acid, ferulic acid, and scopoletin. All test compounds except chlorogenic acid were completely inhibitory at a 10−3m concentration, resulting in death of the cells prior to completion of the growth cycle. To assess the cellular effects of two commonly named plant inhibitors, ferulic and cinnamic acids, these compounds were provided to cultures during incubation of cells with glucose-UL-14C. Incubation of cells with glucose-UL-14C in the presence of 10−4m ferulic acid resulted in increased incorporation of 14C into the soluble lipid fraction along with decreased incorporation of 14C into protein, organic acids, and soluble amino acids. Treatment of the cells with 10−5m cinnamic acid during the incubation period resulted in a significant decrease in incorporation of 14C into protein. These alterations in the flow of carbon into cellular constituents when cells are treated with cinnamic and ferulic acids explain, at least in part, why these compounds inhibit growth, seed germination, and seedling development.