Effects of passive tension on unloaded shortening speed of frog single muscle fibers

Abstract

Experiments were performed to determine the influence of sarcomere length and passive tension on the velocity of unloaded shortening (Vu) as measured by the slack test technique. Slack test results were obtained from intact twitch fibers isolated from the frog (Rana temporaria). Measurements were made both in the absence and presence of passive tension using two different protocols. In one, all releases were initiated from the same sarcomere length and passive tension level; in the other, all releases ended at the same sarcomere length. In the absence of passive tension, no difference was observed between the results from the two slack test protocols. When passive tension was present, performing all releases from the same initial sarcomere length and passive tension level resulted in linear step size-slack time relationships in which the slopes (Vu) were independent of length over a sarcomere length range extending to 3.1 microns, and the intercepts increased with increasing sarcomere length. Performing all releases to the same final sarcomere length in the presence of passive tension produced nonlinear step size-slack time relationships. The results presented here show that, in the presence of significant levels of passive tension, the traditional interpretation of the slope of the slack test plot as the constant unloaded shortening velocity is only correct when all length steps are initiated from the same initial sarcomere length and level of passive tension.