Effects of parecoxib on mesenteric microcirculation in a rat model of sepsis

Abstract

Objective To observe the effects of parecoxib on mesenteric microcirculation in sepsis rats. Methods Thirty-two Wistar rats were randomly assigned into 4 groups (n=8): sham operation group(group Sham), sham+10 mg/kg parecoxib group (group SP), sepsis group [group cecal ligation and puncture procedure (CLP)], CLP+10 mg/kg parecoxib group (group CP). CLP was applied to induce sepsis. Rats were injected parecoxib by intraperitoneal at 20 min after CLP or sham operation, injection was repeated every 12 h, twice in all. Rats in sham group and CLP group were given intraperitioneal injection of the same amount of saline. The changes of hemodynamics and blood gas were measured. Moreover, the mesenteric microcirculation, including perfused vessel density (PVD), total vessel density (TVD), proportion of perfused vessel (PPV) and microcirculation flow index (MFI) of rats with sepsis were detected after CLP or sham operation. Results Compared with CLP group, Parecoxib treatment markedly improve hemodynamics, increasing MAP and reducing heart rate in group CP at 2, 4, 6 h and 8 h after modeling (P<0.05). It can effectively improve the blood gas index of sepsis rats, arterial blood pH and PaO2/FiO2 increased significantly, PaCO2 and lactic acid decreased significantly in group CP at 6, 12 h and 24 h after modeling (P<0.05). Parecoxib treatment markedly improve the mesenteric microcirculation and increase the TVD, PVD and MFI in group CP at 6, 12 h and 24 h after modeling (P<0.05). Conclusions Parecoxib sodium treatment may improve mesenteric microcirculation in septic rats, increase the density of TVD, PVD and MFI. Key words: Sepsis; Parecoxib; Microcirculation