Abstract

Objective To observe the effects of parecoxib on the bone metabolism of rats and intervention of propofol.Methods Thirty-two SD female rats were randomly divided into four groups (n =8each):control group,parecoxib group,propofol group and combination group.The rats were given the medicine intraperitoneally twice daily for 7 days after surgery.The bone mineral density (BMD) at the fracture site was measured using dual energy x-ray absorptiometry (DEXA) on the week one,two,five and eight.The level of serum osteocalcin (OC) was determined by using an enzyme linked immunosorbent assay (ELISA) on the week one and two.In the in vitro experiment,osteoblast-like cell line MC3T3-E1 cells were cultured,and divided into four groups:control group,parecoxib group,propofol group and combination group.Each group was sub-divided into three subgroups:1 μmol/L subgroup,10 μmol/L subgroup and 100 μmol/L subgroup.Western blotting was used to detect the expression of bone protection element (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) proteins 3 days later.Results In the in vivo experiment,two weeks after surgery,the BMD in the parecoxib group (0.091 5 ± 0.003 7) was significantly lower than that in the control group (0.111 8 ± 0.006 4) (P < 0.05).At each time point,the OC in the propofol group (907.137 0 ± 109.923 9) at the first week,and 1 036.863 0 ± 15.551 4 at the second week) was significantly higher than that in the other three groups at each time point (P < 0.05for all).Two weeks later,the OC in the combination group,control group and parecoxib group were respectively 951.165 0 ±85.886 3,736.916 7 ±34.687 4 and 813.571 7 ±63.712 2 respectively.The OC in the combination group was significantly higher than that in both control group and parecoxib group (P <0.05).In thein vitro experiment,RANKL protein in the parecoxib group was significantly lower at the concentrations of 10 μmol/L and 100 μmol/L,and that in the propofol group was significantly higher at the concentration of 100 μmol/L than in the control group,and there was no significant difference between the combination group with all the concentrations and and the control group (P < 0.05).OPG protein in the parecoxib group with all the concentrations was significantly lower,that in the propofol group with all the concentrations was significantly higher,and that in the combination group was significantly higher at concentration of 1 μmol/L than in the control group (P < 0.05).Conclusion The use of parecoxib intervenes in OPG/RANKL signal channel,and has an adverse impact on the bone metabolism in rats.Propofol plays a positive role in modulation of OPG/RANKL,and probably alleviates the propofol-induced harmful effects on the bone metabolism. Key words: Parecoxib; Propofol; Bone metabolism ; Bone mineral density; Osteoprotegerin

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