Effects of ovaprim, a commercial spawning inducer, on vasotocin and steroid hormone profiles in the catfish Heteropneustes fossilis: In vivo and in vitro studies

Abstract

Ovaprim (OVP) is used as an effective spawning inducer for artificial breeding of fishes and contains a salmon gonadotropin-releasing hormone analogue and a dopamine receptor-2 antagonist, domperidone. Previously, we have shown that vasotocin (VT) stimulates ovarian final oocyte maturation, hydration, and ovulation through a mechanism involving induction of a steroidogenic shift, favouring the production of a maturation-inducing hormone (MIH). In the present study, we demonstrated that OVP stimulated brain, plasma and ovarian VT levels, suggesting multiple sites of action, apart from its well established role in the induction of a preovulatory LH surge. An intraperitoneal injection of 0.5μL/g body weight of OVP for different time intervals (0, 4, 8, 12, 16 and 24h) induced ovulation as well as increased significantly brain and plasma VT levels in a time-dependent manner. Plasma steroids were differentially altered; the levels of estradiol-17β (E2) and testosterone (T) decreased, and the MIH (17, 20β-dihydroxy-4-pregnen-3-one; 17, 20β-DP) level increased time-dependently. In order to demonstrate whether OVP acts at the level of the ovary directly, in vitro experiments were conducted. The incubation of ovarian slices/follicles with OVP (1, 5 and 10μL/mL) for different time points (0, 4, 8, 12, 16 and 24h) induced germinal vesicle breakdown (GVBD) in a concentration- and time-dependent manner. Ovarian VT increased significantly in a concentration- and time-dependent manner with a maximal increment at 16h. Ovarian T and E2 levels decreased concurrently with the rise in the MIH level, dose- and duration-dependently. The results show that OVP stimulates VT at the brain and ovarian level. The direct OVP-VT cascade has the potential to stimulate FOM and ovulation, sidelining the pituitary glycoprotein hormone (LH) surge.